Phytochemical assessment, antiradical and antifungal activity of a recipe composed of three plants: Khaya senegalensis, Mangifera indica and Ocimum canum.

Ablam Alognon , Sossawè Poli , Minyo Ega Sossa Montant , Yao Hoinsou , Luckman Gbati , Efui Holaly Gbekley , Akouétévi Gérard Toudji , Bouraïma Djeri

ABSTRACT

Objectives: The present study assessed the pharmacological properties of plants and a recipe used in traditional medicine for the treatment of Candida albicans vaginitis in Togo. Methodology and Results: Qualitative phytochemical analysis was studied using standard tests, Total phenol content, Condensed tannin levels, flavonoid content were determined respectively by the Folin-Ciocalteu method, Butanol-HCl method, and the aluminium chloride method, and free radical scavenging activity by phosphomolybdate reduction and the FRAP method. The microdilution method coupled with spreading on an agar medium was used for the antifungal tests. Phytochemical analysis revealed phenolic compounds, alkaloids, flavonoids, saponosides, tannins, triterpenes, and sterols. Khaya senegalensis contains more phenols (97.26±0.10 mg AGE/g) and recorded the best free radical scavenging activity with both methods (2.7±0.00 with the FRAP method and 0.08±0.00 mgAAE/g) with the molybdate reduction method (0.08 ±0.00 mg AAE/g). Mangifera indica contains more proanthocyanidins (0.98%±0.00 mgCE/g) and more flavonoids (196.35±5.27 mgAGE/g). Antifungal tests showed that all of the plants and that of the recipe had antifungal activity on the germs tested. MICs and MFCs ranged from 3.125 to 50 mg/ml. The recipe extract was fungicidal on most germs tested. Conclusion and application of results: The results obtained partly justify the traditional use of this recipe in the treatment of Candida albicans vaginitis in Togo.

Keywords: Khaya senegalensis, Mangifera indica, Ocimum canum, vaginitis, recipe, plants.